p igf 1rβ (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
p igf 1rβ
P Igf 1rβ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+igf+1r%CE%B2/Phospho-IGF-I+Receptor+beta+(Tyr1135%2F1136)%2FInsulin+Receptor+beta+(Tyr1150%2F1151)+Rabbit+mAb/pm41486508-45-144-149
Average 96 stars, based on 430 article reviews
P Igf 1rβ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+igf+1r%CE%B2/Phospho-IGF-I+Receptor+beta+(Tyr1135%2F1136)%2FInsulin+Receptor+beta+(Tyr1150%2F1151)+Rabbit+mAb/pm41486508-45-144-149
Average 96 stars, based on 430 article reviews
p igf 1rβ - by Bioz Stars,
2026-09
96/100 stars
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Control:Article Title: A Novel Serum-Based Bioassay for Quantification of Cancer-Associated Transformation Activity: A Case–Control and Animal Study Article Snippet: Western blot analysis was performed by quantifying protein concentrations using Bradford reagent (Bio-Rad, #500-0006, Hercules, CA, USA), denaturing 30 μg of protein per sample in 4× Laemmli buffer at 95 °C for 5 min, followed by separation via SDS-PAGE and transfer to nitrocellulose membranes. .. Membranes were probed overnight at 4 °C with primary antibodies against c-MET (BS-0668R, Bioss, Wuhan, China), phospho-c-MET (#3077S), IGF-1Rβ (#3027S), Incubation:Article Title: A Novel Serum-Based Bioassay for Quantification of Cancer-Associated Transformation Activity: A Case–Control and Animal Study Article Snippet: Western blot analysis was performed by quantifying protein concentrations using Bradford reagent (Bio-Rad, #500-0006, Hercules, CA, USA), denaturing 30 μg of protein per sample in 4× Laemmli buffer at 95 °C for 5 min, followed by separation via SDS-PAGE and transfer to nitrocellulose membranes. .. Membranes were probed overnight at 4 °C with primary antibodies against c-MET (BS-0668R, Bioss, Wuhan, China), phospho-c-MET (#3077S), IGF-1Rβ (#3027S), Article Title: A-kinase anchoring protein BIG3 coordinates oestrogen signalling in breast cancer cells Article Snippet: The cells were lysed with lysis buffer (50 mM Tris-HCl; pH 8.0, 150 mM NaCl, 0.1% NP-40 and 0.5% CHAPS) containing 0.1% protease inhibitor cocktail III (Calbiochem, 539134). .. The lysates were electrophoretically separated, blotted onto a nitrocellulose membrane, and blocked with 4% BlockAce solution (Dainippon Pharmaceutical, UK-B80) for 1 h. The blots were subsequently incubated with antibodies against the following proteins: BIG3 (1:1,000, ref. ), phospho-BIG3 (S305, 1:500); phospho-BIG3 (S1208, 1:500), and phospho-PHB2 (S39, 1:500); PHB2 (1:500) (Abcam, ab15019); PKAα cat (C-20, 1:1,000, sc-903), PKCα (H-7, 1:500, sc-8393) and PP1Cα (FL-18, 1:500, sc-443) (Santa Cruz Biotechnology); ERα (SP1, 1:500, RM-9101-S0), phosphoserine (1:1,000, 61–8100), and phosphothreonine (1:1,000, 71–8200) (Thermo Fisher Scientific); IGF-1Rβ (1:500, #3027), Article Title: Severe Insulin Resistance Alters Metabolism in Mesenchymal Progenitor Cells Article Snippet: .. Blots were incubated with primary antibodies, including anti-INSRβ (sc-711; Santa Cruz Biotechnology, Inc), IGF-1R (sc-713; Santa Cruz Biotechnology, Inc), Membrane:Article Title: A-kinase anchoring protein BIG3 coordinates oestrogen signalling in breast cancer cells Article Snippet: The cells were lysed with lysis buffer (50 mM Tris-HCl; pH 8.0, 150 mM NaCl, 0.1% NP-40 and 0.5% CHAPS) containing 0.1% protease inhibitor cocktail III (Calbiochem, 539134). .. The lysates were electrophoretically separated, blotted onto a nitrocellulose membrane, and blocked with 4% BlockAce solution (Dainippon Pharmaceutical, UK-B80) for 1 h. The blots were subsequently incubated with antibodies against the following proteins: BIG3 (1:1,000, ref. ), phospho-BIG3 (S305, 1:500); phospho-BIG3 (S1208, 1:500), and phospho-PHB2 (S39, 1:500); PHB2 (1:500) (Abcam, ab15019); PKAα cat (C-20, 1:1,000, sc-903), PKCα (H-7, 1:500, sc-8393) and PP1Cα (FL-18, 1:500, sc-443) (Santa Cruz Biotechnology); ERα (SP1, 1:500, RM-9101-S0), phosphoserine (1:1,000, 61–8100), and phosphothreonine (1:1,000, 71–8200) (Thermo Fisher Scientific); IGF-1Rβ (1:500, #3027), Article Title: Neuroprotective levels of IGF-1 exacerbate epileptogenesis after brain injury Article Snippet: Protein content of culture lysates was measured using Micro BCA protein assay kit (Thermo Scientific). .. Proteins were separated via electrophoresis in 12% Tris-Glycine Mini Gels (Life Technologies), transferred to a PVDF membrane, and stained with antibodies (1:10,000 rabbit antibodies to phospho-Akt (Ser473) (D9E), phospho-Akt (Thr308) (C31E5E), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E), phospho-S6 (Ser235/236) (D57.2.2E), phospho-S6 (Ser 240/244) (D68F8), FLAG-tag:Article Title: A-kinase anchoring protein BIG3 coordinates oestrogen signalling in breast cancer cells Article Snippet: The cells were lysed with lysis buffer (50 mM Tris-HCl; pH 8.0, 150 mM NaCl, 0.1% NP-40 and 0.5% CHAPS) containing 0.1% protease inhibitor cocktail III (Calbiochem, 539134). .. The lysates were electrophoretically separated, blotted onto a nitrocellulose membrane, and blocked with 4% BlockAce solution (Dainippon Pharmaceutical, UK-B80) for 1 h. The blots were subsequently incubated with antibodies against the following proteins: BIG3 (1:1,000, ref. ), phospho-BIG3 (S305, 1:500); phospho-BIG3 (S1208, 1:500), and phospho-PHB2 (S39, 1:500); PHB2 (1:500) (Abcam, ab15019); PKAα cat (C-20, 1:1,000, sc-903), PKCα (H-7, 1:500, sc-8393) and PP1Cα (FL-18, 1:500, sc-443) (Santa Cruz Biotechnology); ERα (SP1, 1:500, RM-9101-S0), phosphoserine (1:1,000, 61–8100), and phosphothreonine (1:1,000, 71–8200) (Thermo Fisher Scientific); IGF-1Rβ (1:500, #3027), Electrophoresis:Article Title: Neuroprotective levels of IGF-1 exacerbate epileptogenesis after brain injury Article Snippet: Protein content of culture lysates was measured using Micro BCA protein assay kit (Thermo Scientific). .. Proteins were separated via electrophoresis in 12% Tris-Glycine Mini Gels (Life Technologies), transferred to a PVDF membrane, and stained with antibodies (1:10,000 rabbit antibodies to phospho-Akt (Ser473) (D9E), phospho-Akt (Thr308) (C31E5E), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E), phospho-S6 (Ser235/236) (D57.2.2E), phospho-S6 (Ser 240/244) (D68F8), Staining:Article Title: Neuroprotective levels of IGF-1 exacerbate epileptogenesis after brain injury Article Snippet: Protein content of culture lysates was measured using Micro BCA protein assay kit (Thermo Scientific). .. Proteins were separated via electrophoresis in 12% Tris-Glycine Mini Gels (Life Technologies), transferred to a PVDF membrane, and stained with antibodies (1:10,000 rabbit antibodies to phospho-Akt (Ser473) (D9E), phospho-Akt (Thr308) (C31E5E), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E), phospho-S6 (Ser235/236) (D57.2.2E), phospho-S6 (Ser 240/244) (D68F8), Concentration Assay:Article Title: Neuroprotective levels of IGF-1 exacerbate epileptogenesis after brain injury Article Snippet: Protein content of culture lysates was measured using Micro BCA protein assay kit (Thermo Scientific). .. Proteins were separated via electrophoresis in 12% Tris-Glycine Mini Gels (Life Technologies), transferred to a PVDF membrane, and stained with antibodies (1:10,000 rabbit antibodies to phospho-Akt (Ser473) (D9E), phospho-Akt (Thr308) (C31E5E), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E), phospho-S6 (Ser235/236) (D57.2.2E), phospho-S6 (Ser 240/244) (D68F8), other:Article Title: Severe Insulin Resistance Alters Metabolism in Mesenchymal Progenitor Cells Article Snippet: Western Blot:Article Title: Identification of a Novel Invasion-Promoting Region in Insulin Receptor Substrate 2 Article Snippet: .. The following antibodies were used for immunoblotting and immunoprecipitation: IRS2 (4502; Cell Signaling, Danvers, MA), p85 (05-212; Millipore, Billerica, MA), HA (11867423001; Roche), Immunoprecipitation:Article Title: Identification of a Novel Invasion-Promoting Region in Insulin Receptor Substrate 2 Article Snippet: .. The following antibodies were used for immunoblotting and immunoprecipitation: IRS2 (4502; Cell Signaling, Danvers, MA), p85 (05-212; Millipore, Billerica, MA), HA (11867423001; Roche), |
![<t>IGF</t> and HGF signaling mediate cancer serum-induced AIG in TY cells. ( A ) TY-AIG colony formation in response to human ovarian cancer (OvCa) serum or mouse ID8 tumor serum, with or without IGF-1R inhibitor (PPP, 100 nM) or c-MET inhibitor (AMG337, 10 µM). Data shown as mean ± SD ( n = 3); * p < 0.05, ** p < 0.01 vs. untreated control by two-way ANOVA. ( B ) Western blot analysis of IGF-1R, c-MET, and AKT phosphorylation in TY cells treated with pooled ovarian cancer serum ± inhibitors IGF1Ri: 100 nM [PPP]; cMETi: 10 µM [AMG377]. β-actin served as loading control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5745/pmc12345745/pmc12345745__diagnostics-15-01975-g006.jpg)